Anaerostat (anaerobic jar)

Anaerostat (anaerobic jar) - the device for cultivation of microorganisms under aerobic conditions. There are anaerostat normal (Fig. 1) and portable (microbiota). Microorganisms are grown in A. at a given temperature, mostly 37C, under vacuum conditions. Ordinary A. is a rectangular metal box inside which is a thick-walled pot cylindrical shape, closed hermetically. Box has double sides and double bottom, with the space between them filled with water that is removed through a tap on the back A. anaerostat (anaerobic jar) is heated by electricity, constant temperature is provided by thermostat. On the upper panel A. set the gauge and two cranes that can be connected to a vacuum pump.
When growing anaerobic microbes (see Anaerobes) lead temperature to 37 deg, placed in anaerostat (anaerobic jar) test tube or Petri dishes, under anaerobic, door A. hermetically closed and vacuum pump are bringing pressure to a certain extent.
Portable A. consists of thick-walled cylindrical metal container with a tightly closed lid, which runs gauge and two cranes. By A. nutrient medium under anaerobic, from the device pump out air and put it in a thermostat at a given temperature.
Except A., for growing anaerobov apply the device of Calera (Fig. 2) glass cylindrical container with a tightly fitting lid. The vessel has crane, which is connected with the vacuum pump. Between pump and cylinder are manometer and a bottle of sulfuric acid chloride or calcium for absorption of water vapor. After pumping air crane close, l the device is placed into thermostat.

Fig. 1. Anaerostat (anaerobic jar).
Fig. 2. The Office Of Zeisler.