Elgama - Condeca reaction

Elgama - Condeca reaction is used to determine early terms of pregnancy, as well as one of the methods of diagnostics if you suspect gallbladder skidding, horionepiteliome. The reaction is carried out on infantile female white mice. Based on the fact that already in the early stages of pregnancy in a woman's body begins to accumulate chorionic gonadotropin (see Gonadotropic hormones, which in large amount excreted in the urine. If such urine injecting underdeveloped sexually mouse under influence in urine hormones genitals of the test animal begin to grow rapidly, there pattern characteristic of the state of Predtechi or heat animal.
Elgama - Condeca reaction allows to determine pregnancy, but does not allow to differentiate uterine and ectopic pregnancy. To avoid confusion, the reaction is inexpedient to hold, with the delay period less than 10 days, as well as 8-10 days after the termination of pregnancy. If the uterine wall there is even a small piece of chorion (incomplete abortion), the development of chorionic gonadotropin supported and Elgama - Condeca reaction remains positive. If ectopic pregnancy occurred detachment of the ovum, Elgama - Condeca reaction could be negative. Therefore, in each separate case it is important to take into account clinical data.
On the eve of the given reaction woman have to warn about the necessity of some restrictions of reception liquid (with a large dilution of urine gonadotropin may not be enough). To study take urine of the day in pre-boiled dishes (bubble). Better urine to take catheter or during urination first portion of the urine to drain, collecting later. Analyzed urine on the same day injected with observance of the rules of aseptics 4-5 infantile females white mice (weight 7-8 g), 2 times a day for 0.5 ml in 10-hour intervals. Injection of repeated for 3 days in a row. Elgama - Condeca reaction molar and horionepiteliome put divorced with urine (in 5-50-100-200 time), because in these diseases in the urine content gonadotropic hormone sharply increased. After 100 hours of laboratory animals slaughtered, reveal and examine macroscopically (doctor). Active participation in the formulation of the reaction can take a specially trained nurse.

Elgama-Condeca reaction (S. Aschheim, Century Zondek) biological response to identify early pregnancy. Based on the fact that during pregnancy in a woman's body produces a significant amount of human chorionic gonadotropin (see Gonadotropic hormones)released in large quantities in the urine. If the urine of pregnant women to enter under the skin childish immature mice, the process of maturing follicles and education of yellow bodies of animals is considerably accelerated.
Technique reaction. Take at least 4-5 white infantile mice weighing 7-8 grams (about 15-20% of the mice died from poisoning caused by the introduction of urine). Urine for the reaction is recommended to take only after the 10th day of menstruation as pregnancy diagnostic urine taken in the first week after a delay, a significant number of errors.
Morning urine (filter optional) injected twice a day is 8 hours. am and 6 hours. in the evening for 0.5 ml for three consecutive days. Experimental animals opened on the 5th day, i.e. over 100 hours. from the start of the experiment. Changes in the ovaries and tubes established on the basis of macroscopic research.
In unclear cases, you can use an additional method of study, proposed by Cedecom: mice ovaries cut, washed with tap water and put on a glass slide, which is tentatively pour a few drops of glycerin; after 10 minutes on the background of pale (the enlightened) ovarian act boldly blood points (bleeding in the follicles)who are dark-brown, almost black.
Under the influence of gonadotropic hormones, introduced with urine, animals comes one of the following reactions.
The first reaction. The horns of the uterus is enlarged, cyanotic, filled with the secret. In the mucous membrane of the vagina - proliferation polygonal cells. In a smear vaginal discharge - stage heat (estrus) or Predtechi (proestrus), in the ovaries - maturing follicles.
The second reaction. The horns of the uterus small. In the mucous membrane of the vagina changes not. In a smear vaginal discharge - dormant phase (SR of NSAID), in the ovaries - hemorrhage follicles (black dots).
Third reaction. In the uterus no changes, horns her small pale. In a smear vaginal discharge - dormant phase (SR of NSAID), in the ovaries - atleticheskaja yellow body that contains the egg.
The first reaction is not specific to pregnancy; the second indicates the possibility of pregnancy, as well as incomplete abortion, delayed abortion, interrupted tubal pregnancy; the third specific to pregnancy (98-98,5% of cases there is the correct answer). You should not ignore the first reaction, as it is in some cases may signal about a very early stage of pregnancy. This repeated researches revealed the second and third reaction. In the cases violated, dying of pregnancy (broken tubal or ectopic pregnancy, delayed abortion and others) after the third reaction comes the second, and then the first.
After childbirth and abortion gonadotropic hormones usually disappear on 8-12-day, and sometimes even before. When threatened and started abortion positive; in case of incomplete abortion in most cases negative, however, if a piece of chorion still retained the link with the uterus, the reaction may be positive. When suspected ectopic pregnancy accurate diagnosis can be made only on the basis of clinical and laboratory research. Hormonal urinalysis may decide the question of the presence or absence of pregnancy, but do not give instructions, where it occurs (in the uterus or outside). Note that in many cases violated pregnancy reaction could be negative. The refusal of the diagnosis of tubal pregnancy only on the basis of negative A.-C. D. is wrong. The diagnosis is delayed abortion put on clinical grounds and fading A.-C. p. In the absence of pregnancy reaction may be positive in the case of persistence of the follicles or cysts yellow body.
Elgama-Condeca reaction is also used for the diagnosis of a molar, horionepitelioma in which the urine out a large number of human chorionic gonadotropin-releasing hormone. In a normal pregnancy in 1 liter of urine contains
5000-30 000 IU gonadotropin, molar in 5-10 times, and when horionepiteliome - 20-50, sometimes even more. Therefore, A.-C. p. in these cases is divorced with urine (in 5-50-100-200 times). After removal of the gallbladder reborn placenta necessarily dynamic study of urine on the content of gonadotropic hormone because A.-C. D. is the control of the future course of the process (development of horionepitelioma!).
If urine is less than 40 000 UNITS of gonadotropic hormone, the diagnosis of a molar unlikely; when the content in the urine of more than 50 000-60 000 IU hormone diagnosis of a molar or horionepitelioma can be considered probable; when detected, 200 000 IU hormone diagnosis can be put almost unmistakable. However, possible negative A.-C. R. (positive or only with undiluted urine) molar (Asheim, Zondek) and, on the contrary, detection during normal pregnancy 150 000-200 000 IU gonadotropic hormone in 1 l of urine (A. E. Mandelstam, and E. M. Kaplun). Therefore, the diagnosis of horionepitelioma it is necessary to consider not only the result of A.-C. R., and clinical features of disease, and the results of the histological examination of a scraping mucous membrane of the uterus.
Elgama - Condeca reaction in urology apply for suspected tumour of the testis. Zondek watched patients with testicular teratoma, in the urine which was discovered gonadotropic hormone - prolan. During the study of tumors it also noted prolan. A.-C. R. positive tumors of the testis in approximately 30% of patients, which reduces its value as a diagnostic method. The closer testicular tumor is to embryonic type, the more often the reaction is positive. Negative reaction does not eliminate tumors of the testis. Most often negative reaction happens when seminoma, positive, with horionepitelioma. A.-C. R. for tumors of the testis may have prognostic value in cases where before treatment she was positive. Reaction allows to reveal hidden metastases after removal of the primary tumor and depending on its further changes during treatment to foresee the result of the disease.