Bacteriological laboratory

Bacteriological laboratory - scientific institution performing bacteriological, immunological and other microbiological research. There are medical, veterinary and industrial bacteriological laboratory.
Medical bacteriological laboratory organized at hospitals, sanitary and epidemiological stations, etc. for research in order to clarify the diagnosis and sanitary-epidemiological control. Bacteriological laboratory are: Srebarna, sink, preparatory, sterilization and vivarium. The device and the equipment of bacteriological laboratories should be adapted to perform research in sterile conditions which will ensure the personnel from possible infection. The room bacteriological laboratory should be bright and spacious. It is necessary to avoid draughts. A special place is given for colouring preparations.
Compulsory equipment of a workplace of bacteriologist are burner, Bank with a solution of carbolic acid for used pipettes, closed vessel for wool, racks for test tubes and bacterial loop, enameled pans, tweezers, scissors, knife, subject and integumentary glass. In the bacteriological laboratory should have a metal trays Petri dishes, galvanized buckets or pots to reset the used dishes. In addition to the usual laboratory glassware, bacteriological laboratory is supplied with a special kinds of dishes: Petri dishes for growing bacteria in dense environments, bacterial mattresses and other Necessary also rubber bulb for namasivaya in pipettes especially dangerous material. Bacteriological utensils should be washed clean, sterilized by heat treatment and closed sterile cotton plugs. For sterilization of glassware should not be used chemicals, as their trace amounts can affect the development of microbes. The most important for bacteriological laboratories are fit for cultivation of microorganisms (bacterial loop, Pasteur pipettes, glass and platinum spatulas). For carrying out of crops in aseptic conditions bacteriological laboratory equipped with special glass boxes equipped ultraviolet lamps (see Boxes bacteriological).
Bacteriological laboratory: refrigerator for storage of bacterial cultures, serums and other biological substrates; microscope with illuminator; centrifuge; thermostat or thermostat room for growing bacteria; apparatus for shaking various mixtures; autoclave, dry-air sterilizer (oven Pasteur) for sterilization of nutrient media, utensils and electric sterilizers. Utility room to work with laboratory animals, for washing and drying dishes, for filling of nutrient media, etc. have to be properly equipped.
When working in bacteriological laboratories, especially with pathogenic microorganisms, requires observance of the following rules.
1. All persons in bacteriological laboratories, should be bathrobes.
2. Indoors is prohibited eating and Smoking.
3. Every employee should use only their jobs.
4. All operations must be conducted with observance of the rules of sterility: all crops is carried out near the flame, transfusion of contaminated liquids produced above the tray with a disinfectant, etc.,
5. All equipment that come into contact with infectious material, subject to sterilization or destruction.
6. All cultures and infected animals are taken into account and are logged by a special form.

Bacteriological laboratory - scientific institution performing microbiological research. Clinical diagnostic B. L. at hospitals depending on the profile of past conduct research necessary to establish or clarify the diagnosis. Bacteriological laboratory at the San.-the disease. stations conduct preventive examination of the population and sanitary-bacteriological testing of food products. Narrowly specialized B. L. performing control functions, such as companies manufacturing bacterial drugs. In addition to health, there is a network of veterinary B. L. performing diagnostic and preventive animal studies, and specialized B. L. serving the needs of the food industry, agriculture and other Various research tasks decide B. L. in the respective research institutes.
The part of bacteriological laboratories include: laboratory, Srebarna, washing, preparatory, sterilization and vivarium. The premise B. L. should be bright and spacious. Over the washbasin strengthen the bottle with a solution for disinfection of hands. Desktops are covered with linoleum or glass. On the table, put a gas or alcohol burner, the Bank for used pipettes with a 3% solution of carbolic acid, a vessel for wool, bacterial loop, set bacterial standards, racks for test tubes, cuvettes, tweezers, scissors, knife, subject and integumentary glass. B. L. shall be equipped with tanks to clear infected dishes. Usually B. L. arrange special table for colouring preparations.
In B. L., besides the usual chemical utensils, the necessary special dishes: glass Petri dishes (Fig. 1) bacterial culture on solid media; bacterial mattresses (Fig. 2) to obtain large quantities of microbial mass; tubes Ru with hauling for cultivation of bacteria on the shoals of potatoes; wassermans tube length 90 mm inner dia. 9 - 10 mm for the production RAC and agglutination reaction; precipitation tube length 90 mm dia. 3-5 mm; bacterial test tubes for cultivation of bacteria on liquid and dense nutrient mediums; Pasteur pipettes (Fig. 3); pipettes Mora for the planting of infected fluid material; automatic pipettes or pipettes with pears, excluding Nastavenie material mouth. The utensils used in B. L., must be leached in 1-2% solution of Hcl and sterilized at high temperature. The crops in dense nutrient mediums are produced using glass spatulas (Fig. 4) and bacterial loops (Fig. 5). Grow bacteria in thermostats or thermostat rooms.


Bacteriological laboratory, engaged in the study of anaerobic bacteria, shall be provided with anaerostat, vacuum pumps, which are also used for filtering and ultrafiltrate. For observance of sterility in work with bacterial cultures B. L. equipped with a special glass boxes. All of the nutrient medium for bacterial culture, serum stored in the refrigerator.
Each B. L. should have a centrifuge, a machine to shake (Fig. 6) and the microscope. For most studies use a microscope MBI-1 with illuminator OI-7 and phase-contrast device.
B. L. should be equipped by equipment for sterilization: autoclave, office of the Koch, oven Pasteur, a unit for the coagulation of whey. For sterilization of liquid substrates using bacterial filters (see). In B. L. should be fit for filling environments (Fig. 7), sets of reagents for carrying out certain chemical analyses, as well as the comparator Michaelis or potentiometer for determination of pH environment.
Work with the animals in the bacteriological laboratory is carried out only in vivarium (see).
While working in B. L. you must observe the following rules: 1) all located in the laboratory must be bathrobes; 2) every employee should have a work place; 3) B. L. it is forbidden to take food and Smoking; 4) when working with infectious material should use instruments; tools that come into contact with infectious material, be sure to sterilize; 5) when the suction of liquid material it is recommended to use the pear; all pipettes should be plugged with cotton wool; 6) transfusion of infected fluids produced above a vessel with the disinfectant solution; 7) work with the infected material have a burner, burning the edges of the tube, loops, spatulas, and so on; 8) the utensils with an infected material is obligatory labeling indicating the name of culture, the number and date; 9) in contact with infectious material on surrounding objects disinfected - fill this place with disinfectant solution and burn swab with a burning alcohol; 10) infectious materials, utensils register, put in tanks and sterilized the same day; 11) culture store in agar columns in sealed tubes with labels; 12) registration of all cultures, and infected animals is conducted in the journal by a special form.