The method Burri

The Burri method - method of preparation negative bacterium preparations. Drop the studied culture mixed on a slide with a drop of ink, make a thin film (by the method of preparation of smears of blood), dried and mikroskopiruyut flow. Microscopic picture: on a black background visible light unpainted microbial cells. Ink should be diluted with distilled water (1 : 10) and remove coarse particles by centrifugation. Instead carcasses can be used nigrosine, Congo red, acid fuchsin and other dyes. Note that the bacteria in the product remain before it was killed, and can be a source of infection. The Burri method is used mainly to detect pale Treponema of syphilis (see). Cm. also Bacteriological examination.