Functional diagnostics of liver

The study of carbohydrate metabolism. Test with galactose. After taking galactose estimate the speed of its transformation into liver glycogen. The estimation is made on the speed of removal from the blood (see Galactosemia) and the number allocated to the urine (see Galactosuria). A positive test is noted in both acute and chronic diseases P. Sample does not reflect the initial function changes P.
Study of activity of enzymes of blood serum. The majority of studied enzymes in the clinic, the so-called principal series of enzymes (transaminases, dehydrogenase, isomerases, phosphatase, cholinesterase, and others), belongs to the cells of the tissues of all bodies, and therefore their appearance in the blood is not strictly specific for liver disease.
To determine transaminases use enzymatic (spectrophotometric) or chemical (colorimetric methods of determination. Normal for glutamicoxaloacetic (ACT) and glutamicoxaloacetic transaminase (ALT) are considered values, using a modified method, ie, Pashino, 40 and 35%, the ratio of AST/ALT levels in the range of 1.3 to 1.4. The increase in transaminaz in the serum indicates the presence of destructive necrotic processes in the cells of P. and less reflects its functional ability. This increase is observed in the early stages of acute hepatitis (already in predzheltushnogo period) and protracted hepatitis (at transition of chronic hepatitis to cirrhosis). Moderate increase is observed in chronic diffuse liver diseases. Has the value of research in transaminaz in the serum in the treatment of steroid hormones (as an indicator of treatment efficiency).
The definition of aldolase. In normal serum activity of aldolase is 5-8% of Its activity increases already in predzheltushnogo period Botkin's disease, as well as in the early period bestellung forms. During the transition-Botkin's disease chronic sometimes there is a long or wavy moderate increase in the activity of aldolase. Method is used for early diagnosis of Botkin's disease and has a clinical and epidemiological significance.
Alkaline phosphatase. Alkaline phosphatase activity is determined by the hydrolysis of glycerol sodium. In healthy people alkaline phosphatase activity ranges from 1.5 to 5.5 per 100 ml blood serum (by the method of Bogunskogo). The most frequently observed increased activity of alkaline phosphatase and jaundice. A significant increase of enzyme activity is observed in mechanical jaundice with full and long obstruction of the common bile duct. In hepatic jaundice is the most significant increase in the activity of alkaline phosphatase in the presence of intrahepatic obstruction (cholangiolitis hepatitis, biliary cirrhosis). High enzyme activity is observed in the number of liver diseases without jaundice (metastatic and primary carcinoma P., amyloidosis, and others).