Chamber of accounts

camera Bunkera
Fig. 1. Camera Bunkera (bottom section).

Camera calculating devices for counting uniform of blood, cerebrospinal fluid, and microorganisms. Currently used almost exclusively the counting chamber type Bunkera (Fig. 1). It is a glass plate, the middle part (strip) below and isolated grooves from the side strips. At laying on the counting chamber and fixation cover glasses it is based on the acting side strips, and the distance from the average strip to cover adds depth to the camera. On average strip engraved one or two accounts grid-separated groove and consisting of squares of certain size. Knowing the area of a square, the depth of the camera and the degree of dilution of the liquid filling the camera, you can calculate the number of formed elements.


Fig. 2. Net Goryaeva.

Fig. 3. Net Fuchs - Rosenthal.

In the USSR the greatest distribution grid has Goriaev (Fig. 2). It consists of 225 large squares (Piazza 1/25 mm2), 25 of which is divided into 16 small each (area 1/400 mm2). Chamber depth of 0.1 mm.. the red cell count in 80 small squares (5 large). When breeding blood 1 : 200 to determine the number of cells in 1 mm3 the result of calculations is multiplied by 10,000.
To count of leukocytes use all 100 big nerazgrablennye squares. When breeding blood 1 : 20 the result is multiplied by 50. At low count of items, such as eosinophils, use the full size of the grid, given that it is 9 mm2and layer fluid over her 0.9 mm3. Grid Bunkera, Neubauer have the same dimensions, but a different location squares. For counting cells in the spinal fluid applied grid Fuchs - Rosenthal (Fig. 3)with an area of 16 mm2 with the chamber depth of 0.2 mm When calculating on 1 mm3 liquid found throughout the grid, the number of cells divided by 3. To calculate microbes use of the accounts chamber with the lower depths (0.02 mm). At present the accounts chamber are displaced by the automatic devices of the account of formed elements.