L-form bacteria

L-form bacteria (synonym L-options bacteria). The ability to turn into an L-shaped or L-transformation inherent in all types of bacteria. Currently, L-forms received also the Treponema pallidum. Regardless of the species of bacteria, L-forms are formed under the influence of that block some parts of biosynthesis of cell wall, and while braking division of bacterial cell, while maintaining its growth. The ability to L-transformation depends on the origin of cultures, their degree of sensitivity to the transformative impact of individual characteristics of strains and certain cell population.
The formation of L-forms may take the form of direct initial reactions to the action of the transforming agent or be in the final phase changes, which at the time of initial contact with the transformative agent manifested in the forms of heteromorş growth or subcellular forms type spheroplasts. L-transformation is completed education typical L-colonies, which are subdivided into two types - "SV" and "FOR".
The colony "SV" - the size of 0.5-2 mm, mucous consistency, with lace region, densely growing into the environment, pigmented centre. They consist of a spherical bodies of different sizes and optical density, filiform and vakuolyarnyi forms containing inclusions in the form of pellets; in their structure there are also loose granules, form the center of the colony. L-elements colonies "SV" keep the cell wall (albeit in a modified form) and antigenic relationships with bacteria initial view.
The colony "FOR" significantly less (up to 0,5 mm), dry consistency, whitish with a soft lace edge and dense, hard unstable welded with the environment centre. They also contain spherical and vakuolyarnyi body of different size and composition with inclusions in the form of submicroscopic granules. Their composition is dominated by submicroscopic structure, which forms the Central part of the colony, there are many structures uncertain, all the time changing the configuration. The origin of these structures connected with vihodnie cell contents, due to the increase in permeability or destruction of bacterial wall.
L-elements colonies "FOR" largely deprived of cell walls and partly lose the antigenic composition of the original bacterial forms. Colony morphology and microscopic structural elements of L-colonies are so characteristic that may be considered typical characteristic differentiating L-form bacteria from other forms of variability of bacteria. The formation of L-colonies type "SV" and "FOR" marked almost all known species of bacteria.
L-form bacteria can grow in a variety of ways: by division, by budding and the collapse of the smallest elementary bodies, with the ability to reproduce. Despite the physical and osmotic fragility L-elements, they are very viable and long can be stored without replanting in the environment of cultivation. Are highly resistant to L-transforming agents. The bacteria's ability is long to be saved and perebivaetsya in an L-shape, regardless of the content in the environment L-transformative agent, called stabilization. It is caused by hereditary consolidation L-signs. Recovery from L-F.B. cells of the original species of bacteria called the reversion Directors. It depends on the degree of stabilization of the population of L-F.B., the number of pre passages on the environment with the transformative agent and cultivation conditions. The reversion is reproduced experimentally with the exception of environment transformative agent or its destruction.
The ability to reversion each population of L-forms of heterogeneous as separate individuals of a population have different capacities to reversion. Therefore, even the highly stable cultures can meet rare specimens kept ability to reversion. Morphological manifestations reversion diverse. It is accompanied with the formation of various forms of incomplete division, sometimes similar in morphology with the initial phases L-transformation, segmentwise or otpochkovyvaemsya coli bacterial cells, usually lacks this kind of morphology. Depending on the degree of stabilization of L-forms of the process of reversion accompanied by a more or less pronounced recovery signs source view. For revertants L-forms characteristic increased ability to L-transformation, due to a peculiar state of their cell walls, which responds to the impact unable to cause the formation of L-forms of the bacteria had not previously been subjected L-transformation.
Among L-forms of pathogenic species of bacteria can meet strains, preserving the original level of virulence, characteristic of the parent culture (for example, finds virulent L-forms of V. cholerae, toxigenic strains of L-forms of Clostridium tetani, and others). Almost avirulent strains stable L-form is also not indifferent for an organism, so long as they persist in it, have dermatomicosis action and with a repeated lead to increased response, expressed in the event of a severe, long-term healing sterile abscesses and death. L-forms of some pathogenic species of bacteria have a selective cytopathic effect on the number of tissue culture (see). L-forms are frequently detected in the body when such long flowing pathological processes, as brucellosis, septic endocardit, rheumatism and other These data suggest the possible role of L-F.B. in infectious pathology.