Laboratory methods

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In gynecological practice often have to deal with the study of vaginal flora as to find the cause of disease, when one or other inflammatory processes, and to determine the status of the vaginal content, such as before operational intervention.
Before taking smear for research, patient should not douche, and if you want to explore urethral discharge, you should empty your bladder before the test, because after rinsing discharge urine or disinfecting chemicals bacteria in a smear may be missing.
To study take swabs from the urethra, from the lower and the upper third of the vagina, cervix. Before taking smear should be prepared sterile mirrors, clean slides, sterile cotton balls, spoon Volkmann, globality probe or branches of anatomical tweezers.
The patient is laid on the examination table. Spreading pussy lips with the fingers, spoon Volkmann or globulinam probe take allocation from ucreti and put a thin layer on a glass slide. To receive allocations from the upper sections of the vagina impose mirrors and branches of tweezers take detachable. If you want to take the discharge canal of the cervix, vaginal portion of the cervix is exposed mirrors, exterior Xev wiped a sterile cotton ball and spoon Volkmann or branches of tweezers from the cervical canal is taken in the selection. From the received allocations prepare smears on slides and send for examination.
To determine the cause of disease, sometimes produce seed funding. For this purpose usually use a meat broth, agar, ascitic fluid, etc., Allocation are taken bacteriological loop with observance of the rules of reimbursement, sowed in a test tube with broth or agar in a Petri dish and put in the thermostat. Once sown material will promote the growth of colonies (in 1-2 days), do on slides strokes of grown colonies and examine them under a microscope, and if necessary, make transfers to other special environment.
For the same purposes of diagnosis and choice of treatment and to evaluate the resistance of the body to fight infection each patient should be a study of blood. In gynecology, in particular, is widely reaction erythrocyte sedimentation (ROE). In the normal sed rate in figures 8-10 mm in the first hour (according to the method panchenkova); inflammatory processes it is accelerated, depending on the intensity of inflammation (to 30-60 mm per hour).
In recent years, many clinicians to diagnose uterine cancer recommend the use of the smear examination of vaginal discharge; this can be detected suspicious for cancer complexes cells. Research method vaginal smear is based on the fact that the surface of the tumor continuously rejected the tumor cells, which, mixed with mucus, blood, secret of cervical and uterine glands, accumulate in the rear of the vault.
To signs which give reason to suspect the presence of tumors include: polymorphism (diversity) of cells, the presence of large cells with large pinnatisected nuclei, i.e., with nuclei of different sizes and forms, usually intensely colored, uneven size of cells and nuclei, the large number of mitoses, big lumps, found in the vaginal discharge, consisting of groups of tumor cells. The presence of such typical groups of tumor cells gives more reason for a correct diagnosis.
The main value of a method of diagnostics of cancer by strokes is that it is suitable for mass examination with the purpose of detection during prophylactic examinations of patients suspected to have malignant tumors.
When those or other gynecological diseases shown hormone therapy, it is important to ask for vaginal strokes hormonal ovarian function.