Lymphocytes against cancer

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In experiments on mice LACQUER received within 3 days of cultivation of spleen cells with recombinant IL-2 (activity last HU IU/ml). These cells were injected on the 3-th and 6-th day after tumor transplantation, and for the elimination of metastases - on the 10th and 13th day. Used maximally tolerated dose IL-2 (up to 108 cells for injection, intravenous). At the same time, the animals were injected intraperitoneally to 20 - 25 thousand units IL-2 (3 times a day for 5 days, every 8 hours). On the 13th day conducted a study of the tumor and metastases by 18 days. All mice receiving combination therapy, tumors and metastases were subjected to the opposite development.
One of the difficult issues is the specificity of action LACQUER, because they were obtained from healthy donors, who had no contact with tumor antigens and stimulated nonspecific activator-interleukin. This however meant that the VARNISH-cells directed destroyed only tumor targets and do not interact with normal cells. Apparently, donor LACQUER have cooperated in the body of media tumors with sensitised to tumor antigens lymphocytes and was acquired over a short period of time necessary specificity. Expressed concern that the VARNISH-cells is a link in the development of T-killers. Additional injection of IL-2 has contributed to the reproduction of VARNISH in the bloodstream, but only on the condition of re-introduction. Get LAC in terms of cell cultivation easier than to multiply specific T-killers, but the latter could be introduced in smaller quantities.
Application of LACQUER and IL-2 is the better, the less the weight of the tumor to the beginning of immunotherapy. C. Rosenberg with employees showed the combination of immunological treatment with radiation (up to 3000 R on the mouse). Proof that the development of tumors suppressed it VARNISH, was that the effect was absent in the introduction of animals with tumors are irradiated LAK cells. Although LAK cells were injected intravenously, already in the next few hours they settled mainly in the lymph nodes, regional relative to the location of tumor lesions. Additional injection of IL-2 provided the reproduction of these cells, especially noticeable in the spleen and liver. It was possible, even the application of allogenic LACQUER which did not immediately expelled from the bloodstream of the host (as expected), and destroyed pulmonary metastases. However, in these cases, has increased the threat of the development of the reaction of the transplant (lymphocytes) against the owner, especially if immunoreactivity last damaged by radiation. Introduction donor LAC, as the use of adaptive immunotherapy in combination with chemotherapy, it is very important for clinical application experimentally found approaches to cancer treatment.