Myrurgia

Myrurgia is an aggregate of methods and technical facilities for conducting of micromanipulation in the study of microscopic objects.
There are two areas of myrurgia operations not requiring a significant increase (for example, in the experiments on the early stages of the development of embryos, eggs echinoderms and others); operations on the cells or parts under high magnification with special devices in Cytology, Microbiology and genetics (for example, when studying the regulation of gene activity).

Myrurgia (from the Greek. mikros - small and ergon - action) - a set of methods and technical means, which gives the opportunity to perform operations on very small objects - simple, eggs and embryos, cells and multicellular intracellular structures (the kernel, chromosomes).
There are two areas of myrurgia: micro operations on a relatively large objects (eggs, embryos), which are produced under the control of the naked eye or under preparovanie the microscope, micro operations on individual cell or its parts, when the impacts are produced under high magnification microscope using a special device - micromanipulator. Micro tools for myrurgia made from glass, shellac, asphalt, some polymers using special microhole; myrurgia use and soluble needle" from glucose and other carbohydrates. Instruments depending on the purpose is shaped in the form of needles, pipettes, microscalpels, mikrosporia, microbial, micropincers., microelectrodes, etc., In 1912
C. S. Carotin created a special unit for localized damage cells in a thin beam of ultraviolet rays. Technology of ultraviolet MicroCal allows local damage to the cells of a beam with a cross section of 1 MK.
In recent years, for the UV of MicroCal started using methods television. Developed the method of microbicide cells to ionizing radiation. Of great interest to microbicide cells can imagine lasers that allows you to receive only the strong light. Now managed under a microscope to focus the laser beam on a plot of diameter 2 MK.
In the implementation of micro-operations operating the camera combine under a microscope with relevant micro tools (see Micromanipulator), then bring in the field of view covering glass with the object and immobilized it.
Myrurgia can be used to resolve a variety of issues of Cytology and Microbiology (the study of physico-chemical properties of individual parts of the cell, the value of its individual components and the entire structure to maintain normal life and others). Myrurgia can be used for the introduction of a single microbial cells in a test tube or animal organism.
Superior achievement myrurgia are the works on removal and transplantation of nuclei, nucleoli, mitochondria and other elements of the cell. So, COPAC (M. Kopac, 1959) in the U.S. has successfully transfer using a micropipette with a diameter of 1 MK nucleoli and viral inclusions from one cell to another. To save operated cells they are placed in special cameras, for example in oil camera, selecting texture, composition, and reaction of environment depending on the properties of the object and the specific requirements of the experience. In the implementation of micro-operations along the way, are studied within the reactivity of a cell and its capacity to recover. The controls are the same cells, but not undergone surgery.