Carbon monoxide forensic against

Carbon monoxide poisoning in most cases is the result of an accident (frenzy - poisoning in the premature closing overheated furnaces, poisoning illuminating gas at malfunction of gas installations in the kitchens, baths, poisoning by exhaust gas in cars or garages, and so on), less frequently, suicide, murder. Carbon monoxide poisoning is often causes suspicion of violent death, so in such cases conduct investigation into the circumstances and cause of death.
Diagnosis of poisoning by carbon monoxide is based on the clinical picture, the data of dissection and study of blood for the presence of carboxyhemoglobin. In acute carbon monoxide poisoning victim can make an impression of a person in a condition of alcoholic intoxication. This should be considered during the examination of drivers of vehicles that may violate traffic rules and even take accidents and disasters in case of poisoning by exhaust gases.
At the dead bodies of persons who died from acute poisoning by carbon monoxide, skin and mucous membranes have a pink hue, many cadaveric spots, blood and internal organs (cuts) bright red; color degree depends on the content of carboxyhemoglobin in the blood. If death occurs after a few hours or days after poisoning, there are changes in the Central nervous system - dystrophic changes in the ganglion cells, bleeding and pockets of softening in the substance of the brain, and degenerative changes in the heart muscle, liver, kidney.
To establish the presence in the blood of carboxyhemoglobin in section table is a sample Hoppe - Seyler: blood on the plate or in vitro mixed with an equal or double displacement 30Vo caustic soda; the blood containing the carboxyhemoglobin, remains bright red, blood does not contain carboxyhemoglobin, quickly turns brown. In laboratory conditions, usually resort to tannic sample and spectral study. Test with tannin (sample of Kunkele - Wetzel): study and control blood is diluted in 5 times with distilled water and mix in test tubes with triple the number of 3% solution of tannin, in the presence of carboxyhemoglobin formed by shaking tubes sediment has Carmine-red, in the absence of carboxyhemoglobin - gray-brown. Spectroscopic investigation: the range of carboxyhemoglobin is similar to the range of oxyhemoglobin and is characterized by two absorption lines between fraungoferovyh lines D and E. For their differences in blood add a reducing agent (mnogoserijnyj ammonium), and the oxyhemoglobin turns into hemoglobin, the spectrum which is characterized by one broad absorption band, the spectrum of carboxyhemoglobin is not changed. Quantitative determination of carboxyhemoglobin in the blood produced gasometrical method van slice, chronometric gas analysis, various modifications of photometric and spectrophotometric methods.
If the corpse was in the atmosphere of carbon monoxide, he could enter it through the skin. Therefore, the blood from the corpse for research on carbon monoxide should be taken from different areas of the body and in conditions that exclude a possibility of contact with air. The non-detection in the blood of carboxyhemoglobin does not exclude the carbon monoxide poisoning, as upon the occurrence of death within a long time after poisoning by carbon monoxide can be released from the body through the lungs.