Experimental study of tumors

For the first time successfully Perevi tumor adult dogs puppies M. A. Novinsky (1876). Since then, for the experimental study of tumors was applied to a large number of different methods and research proceeded in different directions. Currently, you can cause the animals O. a variety of types and locations with different agents.
Transplantation of tumors. There are auto-, hard-, Homo - and watertransportation O.
Autotransplantation - perenivka Acting the same animal from which it originated, but in another place of his body,as a rule, can not and can ensure the success of future inoculation on other animals. The autotransplantation is a kind of model of metastasis.
Strandplatze - transplantation of the tumor to other animals of the same origin, such as mice of the same line. This kind of transplantation has a somewhat lower chance of success than autotransplantation, but usually. Strandplatze held in conditions of tissue homogeneity of the donor and recipient, is an important method to clarify many issues immunology and cytogenetics O.
Gomotransplantation - perenivka tumors in animals of the same species (although not necessarily the same origin). This is the most common method of obtaining the tumor material for different purposes - morphological, biochemical, immunological research, and most importantly to test new methods of treatment, in particular of new drugs.
The most important conclusion from studies of gomotransplantation O. was the idea of the autonomy of the tumor tissue, about her ability to prejustice and grow in a different body, and provided further inoculation - in many organisms for many years. There O., past hundreds of privivok and existing, as, for example, Ehrlich carcinoma, for more than 60 years. Such long-term and repeatedly perelivayuschayasya the tumor is called tumor strain. Morphological, immunological and other properties of the tumor tissue may change considerably in the transplant process.
Gomotransplantation O. mammals is carried out by transfer from one animal to another suspension of cells or small particles of tissue or her small pieces. So inoculation O. prove their cellular nature. However, it was the study of transplantation A. was found to migrate their not only cells but also cell-free filtrate, which served as the basis for the submission of viral O.
Watertransportation tumor - perenivka tumors in animal of another species. This perenivka for a long time failed; later were found conditions conducive to the success of heterologous inoculation. In some cases it is possible when transplanted into the anterior chamber of the eye or the brain tissue. An important role is played by the decrease in the protective forces of the body of the recipient using the x-ray irradiation or injection of cortisone. Successfully implemented heterologous transplantation of neoplasms, and received a number of strains of the Acting person in rats and in cheek pouches hamsters, subjected to the action of x-rays and cortisone.
The explantation of O. is a method of cultivation of tissues outside the body - are widely used in experimental Oncology. Using this method, they studied the histogenesis of many tumors in humans and animals. Of great interest is observed in some cases, the increasing differentiation of human tumor cells (A. D. tymofiivka).
Possible malignant transformation of normal cells in vitro, which can occur under the influence of chemical carcinogenic substances and extracts from tumor tissue, and spontaneously, without any external influences, resulting in only a long-term cultivation of tissues outside the body. Using the method of explantation can long be maintained, and can be obtained new strains of the tumor. Of them are the so-called clonal strains from a single cell. Great is the value of the method of cultivation of tissues in the study of the etiology Acting, in particular when searching for viruses in them.