Ashing methods

Ashing methods (synonym: podagraria, microinsurance, microsiga) - methods for determination of total and distribution of non-volatile when heated inorganic substances into the tissue used in histology.
Fresh or fixed and not containing inorganic compounds liquids (formalin, alcohol) preparations of a thickness of not more than 10-15 MC slowly nakalevu up to 500-600 degrees. Slides with drugs can be heated on the plates of platinum, porcelain or fused silica on the burner; the best results are obtained when ashing in a special furnace in a stream of nitrogen. Cooled drugs covered cover glass and edging the paraffin. Opalennye drugs mikroskopiruyut dark-field or in the incident light; the distribution of ash on drugs (spidogram) compared with the control sections stained with the usual ways.
Ashing methods can identify in the tissues of calcium, magnesium, iron, silicon, sodium, and potassium; application histopathologically methods or analytical electron microscopy allows with a high accuracy to detect small amounts of many minerals. The disadvantages of digestion is that they do not allow to judge about the nature of the relationship of inorganic substances among themselves and with the organic components of tissues, and do not allow to distinguish between structural elements of the tissue from the products of cellular metabolism. Cm. also Histological techniques.