Pshenichnova method

Pshenichnova method (the method of epidermolysis) is designed for experimental infection with pathogenic microbes mosquitoes - vectors of various infections of man. Method Pshenichnova drawbacks methods of Weigle, Sikora and others, as the receipt of infection in the body vector occurs naturally (by mouth); the application of this method allows mass infestation bloodsuckers in different stages of their morphogenesis.
To infect used kormilchi consisting of two screw each other metal disks, between which stretched epidermolysis (Fig) from the finest of the epidermis of the skin of a human corpse, filmed by mild heat treatment. Through the hole in the bottom of the drive kormilchi, i.e. under the membrane, is brought defibrinated blood that contains any virus in the necessary concentration. Kormilchi warmed up and on the surface of the membrane allowed insects that quickly stick, drink blood and become infected.
However, in the experiment with lice method Pshenichnova as a method of Weigle, was only the method of infection, as in the future could not provide the feeding of infected insects. In addition, lice cannot digest the blood of animals and so feed them accounted for donors-people. In 1960, A. C. Pshenichnova with E. G. Naskova, and R. A. Pshenichnova through breeding and selection was bred race of human lice, which is able to eat on laboratory animals. It opened new prospects for experimentation.
Method Pshenichnova is not only a method of studying the development of virus in the body vectors (see), he successfully used also for maintenance of laboratories of different strains of pathogens (ricketsi, spirohet, bacteria) and accumulation of high concentrations of some pathogens. For a number of infections method Pshenichnova - effective way biological agent indication in the material studied.
In the years of the great Patriotic war by the method of epidermolysis was made protivoprotosana vaccine Pshenichnova - raikher, has been widely used. A vaccine prepared from the dead ricketsi of Prowazeki cultivated in the body lice. Vaccinations were carried out three times. The incidence in vaccinated noticeably decreased, and the disease they proceeded much more than unvaccinated.

Kormilchi (disassembled) for the infection of blood-sucking insects.