Puromycin

Puromycin (straitin) is an antibiotic of purine series produced by Streptomyces albo-niger. Synthesized a number of analogues puromycin. The range of biological engineering activities of a wide: in vitro puromycin strongly suppresses gram-positive and some gram-negative bacteria (You. cereus, You. subtilis, Klebsiella pneumoniae, Sarcina lutea, Staphilococcus aureus and others). Bacteriostatic concentration P. equal 2-8 mcg/ml P. active also in respect of the simplest (the trypanosomes, amoeba), the number of worms (pinworms, tapeworms) and cell cultures. Of Trypanosoma most sensitive to P. show Trypanosoma equiperdum and T. evansi, less - So gambiense, So rhodesiense, T. cruzi; almost insensitive to him T. congolense. Action P. on the trypanosomes is manifested both in vitro and in mice. When administered intravenously in mice puromycin moderately toxic (LD50 = 335 mg/kg of body weight of the animal). Puromycin used to treat some forms of Trypanosomosis in humans (Chagas disease, sleeping sickness). Amebacidal activity P. described in experiments in vitro and in Guinea pigs and rats (in the latter case, the effective concentration P. 6 mg/kg). In a clinical setting a positive result was obtained when applying P. (10 mg/kg / day) in combination with tetracycline for uncomplicated forms of the amebiasis. Puromycin inhibits the growth of some tumors in mice (glioblastomas, adenocarcinoma and transplantable leukemia), but ineffective against tumors in humans. In laboratory practice Petrograd was widely spread as the inhibitor of protein synthesis. When exposed to police into the cells of bacteria and animals synthesis of DNA and RNA are not significantly altered, while the protein synthesis sharply suppressed. Cm. also Antibiotics.