Radioisotope methods of research of erythrocytes

Radioisotope methods of research of erythrocytes allow diagnosing various diseases of the blood system, to study the specificity of various cellular structures, to evaluate the usefulness of separate stages of the development of red blood cells and erythropoiesis in General. The application of radioisotopes greatly simplifies the definition of indicators such as the life span of red blood cells, the volume of circulating erythrocytes and other, allows to determine bone marrow daily production of red blood cells, red blood cell reserve of the body and haemolytic (destructive) the ability of the spleen, liver, etc.
For a label of red blood cells use different isotopes and labeled connection, the choice of which is determined by the aims and objectives of the study. Most suitable Cr51, Fe58, some labeled predecessors DNA (H3 - or C14-thymidine, H3 - or C14-uracil and others), and amino acids (C14-glycine, 835-methionine and others).
Overseas use of radioactive phosphorus R in connection with diisopropyldithiophosphate (DFP32). Cr51 (sodium chromate) apply for the label of red blood cells (Mature cells, erythroid number) in the bloodstream. This method can determine the life span of red blood cells, the volume of circulating red blood cells, bone marrow production of red blood cells, and hemolytic activity of organs. To determine the magnitude of radioactivity use scintillation installation (Radioisotope diagnostics).
To assess hemoglobinopathies functions erythroid cells are most comfortable isotope Fe59 (ascorbate iron citrate iron). When studying nucleic exchange, and the time parameters of the mitotic cycle cells of the red line, the speed of their maturing and other use labeled predecessors DNA. Protein function erythroid elements tested at labeled amino acids. Radioactivity compounds labeled Fe59, C14 and S35, can be defined as radiometrically and the method of autoradiography (see). Radioactivity tritium is logged in most cases autoradiographic. The label of red blood cells may be carried out in vivo and in vitro.