The microbiological reactors

Reactors microbiological machines for the cultivation of microorganisms in the production environment for the manufacture of preventive vaccines, anatoxins, producers of antibiotics, etc.
The reactor is a vessel made of stainless steel, which has a shirt for heating steam and cooling water. Inside microbiological reactor are: turbine mixer with beveled ago blades, rotating with the speed of 150-250 rpm welded to the walls of the reactor dividers and a pipe for supply of sterile air. In addition, modern reactors are equipped with measuring instruments and regulators.
Before sowing microbiological reactor is cleaned and sterilized the ferry, then fill in the nutrient medium (up to 3/4 of the volume), which also are sterilized the ferry. In cooled to the desired temperature environment through a siphon make sterile culture grown microbe. Aerobic bacteria are grown with aeration by supply of sterile air through a special siphon, reaching to the bottom of the reactor. Anaerobic microorganisms are grown without aeration.
In the process of growing every 2 hours from a reactor through the siphon take samples for determination of pH, the number of microbial bodies and checking the purity of culture by viewing strokes. Regulation of temperature and gas regime should be done automatically. The term of growth is determined by the type of germ. For growing diphtheria bacteria coliform, whooping cough and some other currently used special microbiological reactors for continuous cultivation. They feed environment and getting ready culture continuously for the entire period of cultivation.
Industrial cultivation of microbes requires the observance of strict aseptic and special rooms.

reactor for cultivation of bacteria
Reactor for cultivation of bacteria: top - longitudinal section; at the bottom cover of a reactor.

Reactors microbiological - devices used for growing large quantities of microbial mass for the manufacture of preventive vaccines and anatoxin. The reactor microbiological (Fig) is a double-walled metal container with capacity from 50 up to 2000 l, stainless steel or aluminium. The inner surface of microbiological reactor polished. Cover with hole for mixer, thermometers, pressure gauge, discharge pipes, fitting for filing environment, sampling, planting, inspection window, connected to the case coupling bolts through providing leak tightness strip. Mixing of the material is carried out with special mixing or barbotirovanie. Some microbiological reactors have provisions for extinguishing foam. Microbiological reactors equipped with gas meters, devices for measuring of temperature, pressure, pH, concentrations of microbial phone Special equipment allows you to automatically maintain in microbiological reactor preset mode.
Before sowing microbiological reactor thoroughly cleaned and sterilized the ferry, pumping it in his shirt, and then served a nutrient medium (usually up to 3/4 of useful volume), which also sterilized submission pair in a shirt of the Russian Museum Nutrient medium cooled to a temperature of cultivation, then using a special siphon, connected with observance of the rules of sterile work with seed fitting, on Wednesday contribute culture grown microbe. Microbes diphtheria and intestinal group, and other aerobes (see) are grown, hareruya nutrient medium supply of sterile air through a special siphon, reaching to the bottom of the reactor. The number of supplied air is measured by a flowmeter. Anaerobes (see) is grown without aeration.
The control of the cultivation (purity of culture, the number of microbial mass, cietoksna) carry out, selecting samples through a special probe is available in the lid of microbiological reactor.
The term of growth is determined by the type of microbe (5-7 hours - for anaerobic microbes 6-7 days - for microbes tetanus). Grown culture pushed out by compressed air from the reactor for further processing (separation, filtering). R. M. sterilized the ferry and washed.
For the cultivation of diphtheria microbe, bacteria coliform group, whooping cough and some other currently used special R. M. continuous cultivation, in which the feed environment and getting ready culture occur continuously throughout the entire period of cultivation.
Care for microbiological reactor is a monthly routine maintenance and repairs; special attention is paid to the integrity of all components and connections, as well as the correct operation of the control and measuring equipment. Production premises with installed R. M., should meet all the requirements of work in sterile conditions. Sterilization of pipelines is done by steam at a pressure of 2 ATM. The room in which is growing in the Russian Museum anaerobov, should be explosion-proof.
Cm. also Bacteriological techniques.