Schiff reagent

Schiff reagent (H. Schiff; synonym taxinterest acid) - reagent for qualitative determination of aldehyde group of organic compounds. Necessary for carrying out of a number of histochemical reaction (response of Falena for the detection of DNA, CHIC-reaction for the detection of different carbohydrates, reaction Bauer to glycogen reaction Kane to identify plasmas).
There are three ways to obtain reagent Schiff. The way de Tomasi is the dissolution of the basic fuchsin in boiling distilled water and add to cooled to 50 degrees filtrate 1 G. hydrochloric acid, and then when cooled to 25 of metabisulfite sodium or potassium. By way of Bargara and de Lamater the solution is basic fuchsin in boiling distilled water is cooled down to 50 degrees and the filtrate add thionyl chloride.
In 1954, Ichikawa and Ogura (O. Itikawa, y-Ogura) offered to prepare reagent Schiff slow transmission of sulphur dioxide (SO2) through cooled and filtered solution of the basic fuchsin in boiling distilled water. When the solution is transparent and is red, the gas stop. The vessel with a solution close and leave overnight in the dark at room temperature. Pale-red liquid discolor the addition of charcoal with subsequent shake for 1 minute. In the first two ways after cooling the solution is kept in the dark for 12-24 hours, then shake for 1 minute with activated carbon and filtered. The filtrate, acquired during storage pink or red colour to the use of no use.
Store reagent is ready Schiff is recommended at temperature 0-4 degrees. At this temperature in a tightly closed bottle reagent Schiff can save your ink valid during 6 months and longer.