The directional influence of nutrition on health indicators

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Taking into account age peculiarities inclusion of radioactive amino acids in a tissue proteins (I.e. Century Larina, 1967; B. Ya. Medovar, 1976) were used in the experiment to determine the effect of dietary protein on protein metabolism in animals of different age. Young and old animals received a semisynthetic isocaloric food rations 9,18 and 36% casein (Yu. G. Grigorov, B. Ya. Medovar, 1976). Research has shown that including 2-14C-glycine in total proteins of various fabrics: young and old rats is different speeds. At that, the greatest specific radioactivity proteins have liver and kidneys, the smallest of the heart and especially the muscles in both age groups of animals. The nature of the diet has validly pronounced effect on the intensity of the pack animal tissue proteins. So, if the specific radioactivity of proteins liver young animals in low-protein (9% casein) and protein (36% casein) rations was lower than in the controls (18%. casein), in old animals she was not changed. A similar relationship is observed in respect of: radioactivity proteins extracted from the tissues of the kidney, heart and muscles. Comparing the intensity of the label will be included in tissue proteins young and old animals at the same food rations, it can be noted that at low and high-protein diets it is significantly higher in the tissues of old animals. With the exception of muscle tissue and tissue of the kidney in low-protein diet (the differences were statistically insignificant). In the group of animals receiving the control diet, installed only the tendency to increase of radioactivity proteins in the tissues of old animals.
The analysis of the obtained data it is necessary to consider that the level of inclusion of radioactive label depends not only on the intensity of protein synthesis, but also other factors, in particular the status of the exchange Fund amino acids (I. N. Bulankin, E. C. Larina, 1960), as delivered to the body 2-14C-glycine is involved in the biosynthesis of protein in equal conditions with the same amino acid exchange Fund. In this regard, in the study include predecessor in tissue proteins were determined its content in a pool of blood and urine. About the content of free predecessor in the tissues were judged by the amount of radioactivity their protein-free filtrates.
The highest level of radioactivity protein-free filtrates observed in young animals, while the old, it was observed at the diet with a high content of protein.
Comparing these results with the data on the specific radioactivity of tissue proteins, it can be noted that in the body of young animals under the influence of high-Protein diet significantly speeds up the process of anabolism and catabolism of nitrogenous substances, while in older animals, the process of adaptation to the increased protein content in the diet significantly slowed down. Expressed the position confirmed by the literature data on the growth of radioactivity protein-free effluents by reducing the use of amino acids in protein synthesis, coming after partial hepatectomy (R. N. Korotkina, etc., 1968).

Limiting protein in the diet of animals was accompanied by the reduction of the specific radioactivity of protein-free filtrates liver, as well as the radioactivity of proteins that were validly pronounced in young animals compared to animals fed the control diet. In addition to the synthesis associated with the renewal of tissue proteins, and synthesis associated with the growth of animals, almost completely discontinued in old age, there are special kinds of synthesis associated with the function of certain organs (I. N. Bulankin, E. C. Larina, 1960). So, in the liver, the synthesis of serum proteins, which in old age remains at a high level and can serve as indicator of the security of the body food proteins. We studied the content of protein in the serum showed that both young and old animals, in all three of food rations, quantity does not change significantly.