Wright's stain

Wright's stain (A. E. Wright) - method staining of smears of blood. For making dye 1% solution of the main methylene blue 0.5%
aqueous solution of sodium bicarbonate is poured in a wide flask (the thickness should not exceed 5-6 cm). The mixture is heated in the machine Koch or on a water bath to a boil and boil for one hour. The liquid is then cooled and filtered. 1 the volume of filtrate add 5 volumes of 0.1% aqueous solution eosin. The resulting rich sediment shake and mix quickly filter. Collected on the filter cake is dried, dissolved in methyl alcohol (0.1 g dye 60 grams of alcohol). Received dye used for coloring the blood smear.
The method of painting : the dry flow blood smear put 2-3 drops of undiluted dye Wright. In a minute, add 2-3 drops of distilled water and continue coloring another 3 minutes Then paint wash off with water, the product is dried strip of filter paper. Cell nuclei get cherry-red color; the dye is also identifying Auroville grain of leukocytes, lymphocytes and particularly clear - monocytes.