Zeissler environment

Zeissler environment (J. Zeissler) - sugar with blood agar for the cultivation and differentiation anaerobov - agents of wound infections. Is prepared as follows: ordinary massoperenosa the agar add 2% glucose (pH=a 7.2 to 7.4) and sterilized in an autoclave at temperature of 110 degrees 30 minutes To a 100 ml melted, chilled up to 45 agar add 10-15 ml of sterile taken of man, a horse or a sheep's blood, mix thoroughly and pour into Petri dishes. Before using cups, you must wait days at room temperature. The basis for determining the type of anaerobic bacteria on the form of growth on the environment Zeissler in anaerobic conditions. Cm. also Differential diagnostic environment, the Nutrient medium.